SRM to measure protein turnover

Lee, A. Y., Yates, N. A., Ichetovkin, M., Deyanova, E., Southwick, K., Fisher, T. S. et al. (2012). Measurement of fractional synthetic rates of multiple protein analytes by triple quadrupole mass spectrometry. Clin Chem, 58(3), 619-627. [PUBMED] Read More...

HeLa cells, Amino acids

Boisvert, FM; Ahmad, Y; Gierlinski, M; Charriere, F; Lamont, D ;Scott, M; Barton, G; Lamond, A (2012) A quantitative spatial proteomics analysis of proteome turnover in human cells. Mol Cell Proteomics 11, M111.011429 [PUBMED] Read More...

Elemental labelling in an alga

Martin, SF; Munagapati, VS; Salvo-Chirnside, E; Kerr, LE; Le Bihan, T (2012) Proteome turnover in the green alga Ostreococcus tauri by time course 15N metabolic labeling mass spectrometry. J. Prot. Res 11, 476-486 [PUBMED] Read More...

Water as turnover label to study mitochondrial dynamics

Kim TY, Wang D, Kim AK, Lau E, Lin AJ, Liem DA, Zhang J, Zong NC, Lam MP, Ping P. (2012) Metabolic labeling reveals proteome dynamics of mouse mitochondria.Mol Cell Proteomics. 2012 Dec;11(12):1586-94. doi: 10.1074/mcp.M112.021162. Epub 2012 Aug 21. Read More...

Dietary supplementation with stable isotope amino acids

Claydon, A.J., Thom, M.D., Hurst, J.L. & Beynon, R.J. (2011) Protein turnover: measurement of proteome dynamics by whole animal metabolic labelling with stable isotope labelled amino acids. Proteomics (in press) Read More...

Perspective review on proteome turnover studies

Claydon, A.C. & Beynon, R.J. (2012) Proteome dynamics: revisiting turnover with a global perspective. Mol. Cell proteomics (in press) Read More...